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Effect of SFK and Ser/Thr phosphatase inhibitors on both PKA substrate and Tyr phosphorylations. Sperm were incubated for 18 h under capacitating conditions in the presence of either different concentrations of SKI606 (1–100 µM; n = 7) (A) or both SKI606 (30 µM) and different concentrations (0.1–1000 nM) of OA (n = 5) (B). In all the cases, sperm protein extracts were analyzed for phosphorylation by western blotting using α-pPKAs or α-pY as first antibodies.
