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. 2013 Apr 29;19(9):570–580. doi: 10.1093/molehr/gat033

Figure 6.

Figure 6

Functional relevance of the SFK/phosphatase pathway. Sperm were capacitated for 18 h in control media (C) or in media containing either OA (100 nM), SKI606 (30 µM) or both inhibitors together (SKI-OA). (A) Spontaneous and progesterone (25 µM)-induced AR were evaluated by staining sperm with FITC-PSA. Results represent the mean ± SEM of four independent experiments. No significant differences among groups were observed in spontaneous AR levels. *P < 0.05. (B) Sperm were incubated under capacitating conditions in control media (C) or in media containing either SKI606 (30 µM), OA (100 nM) or both inhibitors (SKI-OA) for 18 h, then co-incubated with zona-free hamster oocytes in fresh capacitating media for 2.5 h, and finally the percentage of penetrated oocytes determined. As a control, sperm were capacitated in control media and then co-incubated with zona-free oocytes in the presence of the inhibitors for 2.5 h (SKI-OA/2.5 h). Results represent the mean ± SEM of three independent experiments. *P < 0.05.