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. 2013 Aug 19;8:1089–1095. doi: 10.2147/CIA.S46536

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© 2013 Cai et al. This work is published by Dove Medical Press Ltd, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Ltd, provided the work is properly attributed.

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Assay of protein level of interleukin-1β and tumor necrosis factor-α.

Notes: (A) The levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α by enzyme-linked immunosorbent assay in the minocycline treatment group decreased more significantly than in the control model group. *Denotes P < 0.001. (B) The levels of IL-1β and TNF-α by western blotting, as expressed by the densitometry ratio of IL-1β and TNF-α to β-actin (mean ± standard deviation), was more evidently reduced in the minocycline treatment group than in the control model group. *Denotes P < 0.01 versus control model group.

Abbreviations: IL-1β, interleukin-1β; OD, optical density; TNF-α, tumor necrosis factor-α; M4, M6, M8: 4, 6, and 8 weeks after establishment of diabetes; MT4, MT6, MT8: 4, 6, and 8 weeks after minocycline intervention.