(A) eNOS protein and (B) mRNA, as detected by immunohistochemical staining and semi-quantitative RT-PCR, in the pancreata of normal (e.g. LSL-KrasG12D/+ mice that lack Pdx-Cre), KC (330 days of age), KPC, or eNOS-/- KPC mouse (at the mortality endpoint). T, pancreatic tumor tissue. C, cancer cell line derived from the matched tumor.