Loss of Gata3 does not affect the initial generation of CVGs. Double-immunolabeling shows that GATA3 is expressed in the developing CVG. (A–D) Deletion of Gata3 does not significantly affect the generation of CVG neurons as comparable NEUROD+ cells (arrowheads in C) are generated in Gata3-null mice. Similar results are obtained when a different developmental stage (E12.5) were analyzed (E–H). (I–L) At E11.5, the deletion of Gata3 has no detectable effect on the generation of ISL1+ CVGs. (M–P) Further analysis revealed that ISL1+ CVG neurons are not significantly changed at E12.5. OV, otic vesicle; CVG, cochleovestibular ganglion. CD, cochlear duct. D, dorsal; M, medial; V, ventral; A, anterior; L, lateral. Scale bar (A–D) 25 µm. (E–P) 50 µm.