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. 2013 May 13;22(18):3624–3640. doi: 10.1093/hmg/ddt213

Figure 3.

Figure 3.

Functional polymorphism (luciferase reporter) assays: SHR promoter and 3′-UTR polymorphisms decrease Chga gene expression in cultured cells. Luciferase reporter assays were used to determine whether polymorphisms within the SHR Chga promoter and 3′-UTR affect gene expression. Note that WKY and BN promoter and 3′-UTR sequences are identical. (A) Chga promoter. The basal transcriptional activity of the SHR Chga promoter was less than that of the WKY Chga promoter in both rat pheochromocytoma PC12 cells and human neuroblastoma SH-SY5Y cells (*P < 0.05 versus WKY basal expression). (B) Chga 3′-UTR. In PC12 cells, co-transfection of synthetic precursors for microRNAs predicted to hybridize differentially to the polymorphic region in the Chga 3′-UTR revealed a significant (two-way ANOVA) genotype-by-microRNA interaction for miR-22. Data are presented as mean ± standard error. *P < 0.05 versus WKY-negative control. +P < 0.05 versus SHR-negative control.