Table 1.
Polymorphism discovery across the rat Chga locus
| Polymorphism(s) |
Strain allele(s) |
||||
|---|---|---|---|---|---|
| Location | Domain | Type | BN (normotensive) | WKY (normotensive) | SHR (hypertensive) |
| −1694 Ins/Del | Promoter | Ins/Del | – | – | G |
| A-1616T | Promoter | SNP | A | A | T |
| −753 Ins/Del | Promoter | Ins/Del | 15 ‘A’ repeat | 15 ‘A’ repeat | 11 ‘A’ repeat |
| C-177T | Promoter | SNP | C | C | T |
| C-59T | Promoter | SNP | C | C | T |
| +6361 Ins/Del (Gln repeat) | Exon 5 | Ins/Del | 15 tri-nucleotide ‘CAG’ repeats (19 Gln repeat) | 16 tri-nucleotide ‘CAG’ repeats (20 Gln repeat) | 8 tri-nucleotide ‘CAG’ repeats (12 Gln repeat) |
| +8093 Ins/Del (Glu repeat) | Exon 6 | Ins/Del | GAG (16 Glu repeat) | GAG (16 Glu repeat) | – (15 Glu repeat) |
| G + 11177T (G + 174T) | Exon 8 (3′-UTR) | SNP | G | G | T |
A list of polymorphisms discovered through resequencing of the Chga locus in the SHR, WKY and BN rat strains is presented. Polymorphisms are numbered according to their position in relation to the mRNA cap site (transcriptional start site), which was designated as position ‘0’. Base pair position upstream of the cap site (i.e. in the promoter) was numbered negatively in the descending order. Base pair position downstream of the cap site was numbered in the ascending order. Coding region polymorphisms within exons 5 and 6 resulted in in-frame deletions of codons for glutamine and glutamic acid. Polymorphism G + 11177T in Exon 8 is also named G + 174T, which refers to its position relative to the beginning of the 3′-UTR.
Ins/Del, insertion/deletion; SNP, single nucleotide polymorphism; 3′-UTR, 3′-untranlsated region; Gln, glutamine (amino acid); Glu, glutamic acid (amino acid).