Table 4.

Effect of miR-22 antagomir treatment on Chga mRNA and protein abundance in the SHR

Analyte	Brainstem fold change (antagomir/control)	Adrenal gland fold change (antagomir/control)	Plasma fold change (antagomir/control)
Chga protein	1.33-fold (P = 0.027)	0.52-fold (P = 0.01)	No change
Chga mRNA	No change	No change	Not applicable

We measured Chga protein and Chga mRNA transcript abundance fold change in SHR after 9 days of treatment with miRNA-22 antagomir or negative control. Statistically significant P-values (<0.05) are shown in parentheses following fold-change values. Fold change >1 indicates increased abundance in the miR-22 antagomir treatment group; fold change <1 indicates decreased abundance in the miR-22 antagomir treatment group. Data were analyzed with Student's t-test.