Detection of Barr bodies by autoimmune serum 154. Indirect immunofluorescence was performed on diploid human fibroblasts containing different numbers of X chromosomes using autoimmune serum 154 (1:200 dilution) and an FITC-conjugated secondary antibody against human immunoglobulins. Images were collected with a Bio-Rad 1024 ES confocal microscope. (A) GM00468 (46,XY). (B) GM06111 (46,XX). (C) GM00254 (47,XXX). (D) GM01415E (48,XXXX). (E) GM05009C (49,XXXXX). (F) Human-hamster hybrid cell line 8121 (containing an inactive human X chromosome). Arrows indicate Barr bodies.