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Colocalization of XIST RNA and major sites of immunostaining by autoimmune serum 154. Indirect immunofluorescence with autoimmune serum 154 (1:200) followed by RNA FISH with a probe against the XIST RNA were performed on human female fibroblast cells GM00254 (47,XXX). (A) DAPI staining. (B) Immunostaining with serum 154. (C) FISH with probe for XIST RNA. (D) The merged images of B and C.
