Fig. 6.

Estrogen or IFN-α-mediated up-regulation of Unc93b1 expression depends on p202 protein expression. (A) Murine J774.A1 cells that were stably infected with either control lentivirus or the virus encoding shIfi202 were either left untreated or treated with IFN-α (IFN, 1000U ml⁻¹) or estrogen (E2, 10nM) for 14h. After the treatments, total RNA was isolated and was subjected to quantitative real-time PCR using TaqMan assay for the murine Unc93b1 gene. The ratio of the Unc93b1 mRNA levels to β2-microglobulin mRNA was calculated in units. The ratio of mRNA levels in the control cells is indicated as 1. The error bars represent the standard deviation (**P < 0.01; ***P < 0.001; NS, not significant). (B) Murine J774.A1 cells that were stably infected with either control lentivirus or the virus encoding shIfi202 were either left untreated or treated with IFN-α (IFN, 1000U ml⁻¹) or estrogen (E2, 10nM) for 14h. After the treatments, total cell lysates were analyzed by immunoblotting for the indicated proteins. FC, fold change in the Unc93b1 protein levels is indicated.