Figure 3. Lm-elicited γδ T cells display contextual specificity to the priming pathogen.

(A) Mice infected 170 days previously were challenged either orally with 2×10¹⁰ CFU Lm or intravenously with 2×10⁵ Lm. MLNs were harvested at the indicated times following secondary infection to enumerate γδ T cells based on CD27 and CD44 expression. Data is displayed as mean ± SEM and representative of at least two independent experiments of 4 – 5 mice per group. See also Figure S3A-D. (B) Mice which had received a secondary infection were rested an additional 124 days prior to a tertiary oral Lm infection with 2×10¹⁰ CFU and γδ T cells were analyzed for CD27 and CD44 expression 5 days later (red squares represent individual mice). Data is represented as mean ± SEM. (C and D) γδ T cells from the MLN of oral Lm immune mice or oral Lm immune mice rechallenged by oral infection with Lm, S. typhimurium strain BRD509, or S. typhimurium strain SL1344 were analyzed 5 days later for (C) CD27 and CD44 expression and (D) α4β7 expression. Representative data is displayed as mean ± SEM from two independent experiments with 3 – 4 mice per group. See also Figure S3E.