Skip to main content
. 2013 Aug 22;9(8):e1003566. doi: 10.1371/journal.ppat.1003566

Figure 5. The C-terminal region of GPI-PLC contains a flagellar concentration signal.

Figure 5

Cell lines modified to contain a single copy of either wild type T. brucei or T. congolense GPI-PLC, hybrids or the P340G mutant, all modified with a C-terminal eYFP tag, were imaged using the native fluorescence of the eYFP tag after being immobilised. Each image is representative of the distribution observed in the majority of cells. Scale bar represents 2 µm. The schematic on the left shows the hybrids expressed with the T. brucei sequence in grey and the T. congolense sequence in white. The position of the switch in sequence is shown above the hybrid sequence and the single residue mutations are indicated. The exposure time used to image the T. congolense GPI-PLC was greater than the exposure time for the other cell lines due to the low expression level. Scale bar represents 2 µm.