Table 1. Summary of subcellular localisation and GPI-anchor hydrolysis for wild type and mutant GPI-PLC (n.d - no data).
Motif | Cell and flagellar membrane localisation | Percentage of cells with GPI-PLC associated with the endosomal system (%) | Release of soluble VSG on hypotonic lysis | Mean ratio of GPI-PLC on the flagellar membrane to the cell membrane (±sem) |
CCGAC | Y | 29 | Y | 3.8 (±0.3) |
SCGAC | Y | 100 | Y | 1.2 (±0.1) |
CSGAC | Y | 32 | Y | 2.0 (±0.2) |
CCGAS | Y | 100 | Y | 2.1 (±0.3) |
CSGAS | Y | 100 | partial | n.d |
SSGAC | N | 100 | N | n.d |
SCGAS | N | 100 | N | n.d |
SSGAS | N | 0 | N | n.d |