Figure 5. GAG binds to epithelial cells.

(A) Soy Bean Agglutinin (SBA), a GalNAc binding lectin, detects GAG on hyphae. Hyphae were grown for 12 h, fixed, stained with FITC-conjugated SBA and imaged using confocal microscopy. Magnification was 40×. (B) Dose dependent binding of purified GAG particles to A549 cells. Cells were co-incubated with the indicated concentration of GAG, and then washed and bound GAG was quantified by detection with FITC-conjugated SBA. The assay was performed on three independent occasions. Graphs indicate mean ± standard error.