Figure 6. Uge3 is required for the induction of epithelial cell injury by A. fumigatus.

A549 pulmonary epithelial cells were incubated with conidia of the indicated strains for 16, 20 or 24 h, after which the extent of epithelial cell injury was measured with a chromium release assay. Graphs indicate mean ± standard error of 3 independent experiments, each performed in triplicate. * indicates a significantly reduced injury to cells induced by the Δuge3 mutant, as compared with injury induced by Af293 and uge3-complemented strains, p<0.05 by factor ANOVA.