Figure 8. β-glucans are exposed on hyphae in the absence of GAG.

Detection of Fc-dectin-1 binding using indirect immunofluorescence. Conidia of A. fumigatus strains Af293 and Δuge3 were grown in Brian media for the indicated times, to produce swollen conidia (6 h), early germinating conidia (8 h) or hyphae (12 h). Samples were then fixed and stained with Fc-dectin-1 (A) or with aniline blue (B) and imaged with epifluorescent microscopy at 40× magnification. (C) β-glucan content of supernatants harvested from the indicated strains, as determined by the (1→3)-β-D-Glucan Detection Reagent Kit. Results are the mean ± standard error of two independent experiments.