Figure 10. Dectin-1 blockade abrogates the increased TNFα secretion induced by the GAG deficient Δuge3 mutant.

BMDDCs were infected with hyphae of the indicated strains for 6 h, after which the TNFα content of culture supernatants was determined by EIA. Dectin-1 recognition of β-glucan was inhibited by preincubating BMDDCs with a monoclonal anti-dectin 1 antibody or by preincubating hyphae with Fc-dectin-1. Results are mean ± SEM of duplicate experiments, each performed in triplicate. * indicates a significant reduction of TNFα production compared to BMDDCs exposed to the Δuge3 mutant without dectin-1 blocking, p<0.05 by factor ANOVA.