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. 2013 Aug 22;9(8):e1003575. doi: 10.1371/journal.ppat.1003575

Figure 12. The A. fumigatus Δuge3 mutant induces a hyperinflammatory response in non-neutropenic mice that is attenuated in highly immunocompromised mice.

Figure 12

(A.) Corticosteroid treated mice were infected by inhalation with the indicated strains of A. fumigatus and sacrificed three days after infection. Fungal burden was determined by pulmonary galactomannan content and pulmonary inflammation was measured by determining MPO, and TNF-α content. Pulmonary injury was quantified by measuring LDH release in BAL fluid. MPO, TNF-α, and LDH levels were normalized to the fungal burden of each strain in Panel 1. Results are median ± interquartile range of 8 mice per strain. * indicates a significant decrease in fungal burden or a significant increase in MPO, TNFα or LDH content in lungs of mice infected with the Δuge3 mutant as compared to the lungs of mice infected with the wild-type strain, p<0.01 by the Wilcoxon rank sum test. (B) Corticosteroid and cyclophosphamide treated mice were infected by inhalation with the indicated strains, sacrificed and the lungs processed as in (A). MPO, TNF-α, and LDH levels were normalized to the fungal burden of each strain in Panel 1. Results are median ± interquartile range of 9 mice per strain. Note: y-axis values for all graphs are lower than those in (A).