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. 2013 Aug 22;9(8):e1003558. doi: 10.1371/journal.ppat.1003558

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Purified MCV pseudoviruses made with WT VP2 or Gly₂-mutated VP2 (G2F, G2S, and G2V) were analyzed by SDS-PAGE and SYPRO Ruby protein stain. (B) The infectivity of MCV pseudoviruses containing WT VP2 or Gly₂-mutated VP2 was determined in 293TT cells using flow cytometry. The average percentage of GFP-positive cells from four experiments is shown and error bars represent the standard deviation.