Figure 7. LTB₄ production after fungal infection.

(A) Enzyme immunoassay quantification of LTB₄ concentrations in alveolar macrophages cultures after 24 h of incubation with P. brasiliensis yeasts. Data are presented as mean ± SEM and are representative of one experiment (n = 8). Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) when compared to uninfected cultures (only medium) or between indicated groups. Symbol ^† represents significant difference (^†††p<0.001) between WT and 5-LO^−/− macrophages under the conditions mentioned. (B) Lung LTB₄ concentration in WT and 5-LO^−/− mice. Quantification of LTB₄ in lungs from uninfected- (control - NI) or infected mice with P. brasiliensis evaluated 24 h after inoculation. Leukotriene concentrations in supernatant of homogenized lungs (100 mg) were expressed as pg/ml. Data were expressed as the mean ± SEM and are representative of one experiment (n = 6). Symbol * represents significant difference (***p<0.001) when compared to uninfected mice. Symbol ^† represents significant difference (^†††p<0.001) between WT and 5-LO^−/− mice under the conditions mentioned.