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. 2013 Aug 22;7(8):e2398. doi: 10.1371/journal.pntd.0002398

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© 2013 Lau et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Northern blot analyses of small RNA samples in wild-type (WT), dcl-1^KO, dcl-2^KO, dcl^DKO and qde-2^KO strains of P. marneffei showing that the production of milRNA of (A) PM-milR-M1 and (B) PM-milR-M2 requires DCL-2 but not DCL-1 or QDE-2. The ethidium bromide-stained denaturing gel in the bottom panel showed equal loading of RNA. Predicted structures of pre-milRNA of PM-milR-M1 and PM-milR-M2, with their milRNA and paired milRNA* sequences as labeled in red and green respectively, are shown next to the northern blot analyses. The probe sequences used for northern blot analyses are marked.