Table 3. Offspring renal function is unaffected by maternal salt-exposure.

Renal functional parameters at baseline in adult offspring at 8 weeks of age
		± Maternal salt			P value
	sex	−ve	+ve	s.e.d.	Salt	Sex	Salt*Sex
Urine output (ml/min/kg BW)	male	20.0	30.1	5.3	0.07	<.001	NS
	female	33.9	43.3
Osmolal clearance (ml/min/kg BW)	male	0.05	0.07	0.1	NS	0.002	NS
	female	0.11	0.08
Free water clearance (ml/min/kg BW)	male	20.0	27.9	3.3	NS	<.001	NS
	female	34.7	35.2

A 24 h urine collection with paired blood sample enabled analysis of renal function in offspring. Osmolarity and electrolytes were measured by an osmometer (Osmomat 030, Gonotec) and ICP-MS (XSeries II, Thermo Fisher, Ltd), respectively. Data are estimated marginal means plus the standard error of the differences between means (s.e.d.) for: Control diet (−ve salt, n = 7 dams), n = 13/13 male/female offspring; 4% salt (+ve salt, n = 5 dams), n = 6/8 male/female pups. Data were analysed by mixed effects models with treatment (control vs. 4% salt) and sex (male vs. female) or their interaction as fixed effects and dam included as a random term (Genstat v14). NS, not significant.