Table 1. Primer sequences and UPL probes used for real-time PCR.

Gene	Forward (left) primer	Reverse (right) primer	UPL probe†
EGFR/HER1	5'-cagccacccatatgtaccatc-3'	5'-aactttgggcgactatctgc-3'	42
HER2	5'-ccctgacctgctggaaaag-3'	5'-ggccgacattcagagtcaat-3'	43
HER3	5'-acagccccagatctgcac-3'	5'-gttgggcgaatgttctcatc-3'	9
HER4	5'-ttccactttaccacaacatgcta-3'	5'-cagaatgaagagcccacca-3'	78
NRG1	5'-gatcagcaaattaggaaatgacag-3'	5'-ggcataccagtgatgatctcg-3'	53

† Universal probe library (UPL) probe number (Roche, Basel, Switzerland).