Table 1. Primers for amplification of mt DNA genome of P. cervi.
Primer name (positions) | Sequence of primer (5′→3′) |
PC1-F_nad5 (12177–12198) | TTDCKTCTCGNTTBGGKGATGT |
PC1-R_cytb (1497–1519) | ARAAARTAYCACTCNGGCTTWAT |
PC2-F_cytb (1110–1132) | TATTGRGCTGCTACDGTTTTGAC |
PC2-R_nad2 (4623–4648) | CATCATATGACACCAACAATAATACC |
PC3-F_nad2 (4054–4076) | TTTKTTTATGAGRTYTTTGTBGC |
PC3-R_nad1 (5839–5963) | AYTCDCTYTCVGMCTCMSCRTAATC |
PC4-F_nad1 (5350–5372) | CGTAAGGGKCCDAAHAAGGTTGG |
PC4-R_cox1 (7603–7628) | CCAAARAAYCAAAAYAWATGYTGAAA |
PC5-F_cox1 (7285–7307) | GTTGGKTGRACTTTTTATCCWCC |
PC5-R_s-rRNA (9455–9474) | AGATAAGAACCGACCTGGCT |
PC6-F_s-rRNA (9296–9318) | TTACCTYGGGGATAACTRRGTAA |
PC6-R_nad6 (10851–10876) | GCACCACAHAAYTCMSTACARTAHCC |
PC7-F_cox2 (10265–10292) | TAGCTCTGATAAGTCGTAACATGGTAAT |
PC7-R_nad5 (12326–12343) | HGGAGCVCGCATHGCYTC |
Notes: D = A/T/G; H = A/C/T; K = G/T; M = A/C; N = A/C/G/T; R = A/G; V = A/C/G; W = A/T; Y = T/C. The positions of primers in the study are based on the mt genome sequence of P. cervi.