Skip to main content
. 2013 Aug 22;8(8):e72307. doi: 10.1371/journal.pone.0072307

Figure 2. A small molecule screen by rhomboid FluoPol ABPP and subsequent hit confirmation.

Figure 2

(A) Screening data of the 85 compounds investigated in the FluoPol assay. A 15% cut-off was set to select primary hits. For compounds 3, 9, 10, 12 the slightly negative values are depicted as zero. (B) Confirmation of the potential inhibitors using GlpG wild-type (WT) or S201A mutant (M) by gel-based competitive ABPP using probe EK2 (upper panel) and by substrate cleavage. Uncleaved (#) and cleaved (*) substrate is indicated (lower panel). (C) Chemical structures of the verified hit compounds. (D) Chemical structure of the previously identified inhibitor S016.