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. Author manuscript; available in PMC: 2014 Sep 28.
Published in final edited form as: Cancer Lett. 2013 Apr 2;338(2):229–238. doi: 10.1016/j.canlet.2013.03.032

Fig. 1. BKM120 effectively decreases the survival ( A) and induces apoptosis (B–D) of human NSCLC cells.

Fig. 1

A, A panel of human NSCLC cell lines as indicated were seeded in 96-well plates and then treated with different concentrations of BKM120 ranging from 0.125 to 4 µM as indicated on the second day. After 3 days, cell numbers were estimated using SRB assay. Points, means of triplicate determinations; bars ± SD. B–D, The given cell lines were treated with the indicated concentrations of BKM120 for 24 h and then harvested for analysis of annexin V-positive cells with flow cytometry (B), for measurement of histone-associated DNA-fragments with a cell death ELISA kit (C) and for detection of caspase cleavage with Western blotting (D). Columns, means of duplicate (D) or triplicate (E) determinations; bars ± SD. Pro-casp, pro-caspase; CF, cleaved form.