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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Jul 22;191(5):2184–2193. doi: 10.4049/jimmunol.1301221

FIGURE 4. ADAMTS13 deficiency leads to platelet aggregation and C3 deposition on glomerular endothelial cells under flow.

FIGURE 4

Histamine-pretreated PGEC cells were perfused with serum and normal washed platelets. (A) Perfusion of serum from TTP Patient 4 together with normal washed platelets led to platelet aggregation on the endothelial cells (arrowheads). (B) The aggregates shown in panel A stained positively for C3. (C) Endothelial cells that lodge the platelet aggregates differed morphologically and were rounder in shape (arrowhead). The inset shows similar morphological changes from a different region. (D) The cells shown in panel C stained positively for C3 (arrowheads). (E) Perfusion of normal serum with normal washed platelets did not result in formation of platelet aggregates and no C3 staining was visible (F). Images were taken at 200x magnification (A-F), both the insets in panel C and D were taken at 400x magnification. Reproducible results were obtained from three patients.