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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Jul 26;191(5):2837–2846. doi: 10.4049/jimmunol.1300895

Figure 3. Proteasome inhibition by BZ increases IL-8 expression in PC3 cells, while it inhibits expression of other NFκB-dependent genes.

Figure 3

(A) Real time RT-PCR analysis of mRNA levels of Bcl-2, Bcl-3, cIAP-1 and cIAP-2 measured in PC3 cells treated with increasingBZ concentrations for 24 hours. (B) Real time RT-PCR of IL-8 mRNA levels in PC3 cells treated 24 hours with increasing BZ concentrations. (C) Real time RT-PCR of IL-8 mRNA levels in PC3 cells treated with 1 μM BZ for 0, 6, 24 and 48 hours. (D) IL-8 protein release measured by ELISA in cell culture supernatants from PC3 cells treated 24 hours with increasing BZ concentrations. The values represent the mean +/−SE of four experiments; asterisks denote a statistically significant (p<0.05) change compared to control untreated (UT) cells. (E) Real time RT-PCR analysis of mRNA levels of IL-8, Bcl-2 and cIAP-1 in untreated HeLa, PC3, DU145, Hut-78 and U937 cells. The data are expressed as percentage of mRNA compared to HeLa cells, which was considered 100%; the values represent the mean +/−SE of four experiments; asterisks denote a statistically significant (p<0.05) change compared to HeLa cells.