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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Jul 26;191(5):2837–2846. doi: 10.4049/jimmunol.1300895

Figure 5. Proteasome inhibition by BZ increases p65 but not p50 recruitment to the endogenous IL-8 promoter in PC3 cells.

Figure 5

(A) Schematic illustration of the proximal NFκB binding site in human IL-8 promoter and the ChIP primers used in the assay. Recruitment of NFκB p65 (B) and p50 (C) subunits to NFκB-dependent promoters of IL-8, Bcl-2, Bcl-3, cIAP-1 and cIAP-2 genes in PC3 cells treated 24 hours with 0, 0.1 and 1 μM BZ was analyzed by ChIP and quantified by real time PCR. The data are presented as the change in occupancy over the human IGX1A (SA Biosciences) sequence control and represent the mean +/−SE of four experiments. Asterisks denote a statistically significant (p<0.05) change compared to control untreated cells.