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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Immunol. 2013 Aug 2;191(5):2319–2327. doi: 10.4049/jimmunol.1300851

Figure 1. GrB and Spi6 expression in naive and activated T regulatory cells in vitro.

Figure 1

CD4+ T cells isolated from WT and Spi6−/− C57BL/6 mice were stimulated in vitro in a Treg generation assay. (A) Representative histograms of GrB expression in CD4+ regulatory T cells (gated on CD4+FoxP3+) from unstimulated Spi6−/− (red color) and WT (Dark blue) mice at baseline (day 0); and day 3 post-stimulation (Spi6−/−: Light blue and WT: black). Both WT and Spi6−/− Tregs comparably increased their expression of GrB at day 3 as compared to day 0. Bar graph shows the MFI of GrB expression. (*p<0.05; n=3-5 mice; data representative of 3 separate experiments). (B) Representative histograms of Spi6 expression in CD4+ regulatory T cells (gated on CD4+FoxP3+) from WT and mice at day 0 (Spi6−/− : red color and WT : Dark blue) and day 3 (Spi6−/− : Light blue and WT : black). As compared to Spi6−/− Tregs, WT Tregs increased their expression of Spi6 at day 3. Bar graph shows the MFI of Spi6 expression at day 0 and day 3. (*p<0.05; n=3-5 mice; data representative of 3 separate experiments).