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Burst kinetic isotope effects on (A) cefoxitin and (B) meropenem in H2O (black) and D2O (gray) with fits of experimental traces to the k2 (acylation) and k3 (deacylation) values in Table 1 (red). Cefoxitin studies consisted of 1800 μM cefoxitin mixed with 27 μM BlaC. Meropenem studies consisted of 875 μM meropenem mixed with 13 μM BlaC.
