Table 1.

Solvent Kinetic Isotope Effects on BlaC

Substrate	Nitrocefin	Cefoxitin	Meropenem
Km (μM)	86 ± 6	255 ± 9	3.4 ± 0.7 a
kcat (s−1)	38 ± 2	0.7 ± 0.1	(7.7 ± 0.1) × 10−4 a
kcat/Km (M−1s−1)	(4.4 ± 0.1) × 105	(2.7 ± 0.3) × 103	(2.3 ± 0.8) × 102 a
k2 (s−1)	n.a.	1.4 ± 0.01	(1.9 ± 0.3) × 10−2
k3 (s−1)	n.a.	0.4 ± 0.001	(4.0 ± 1.0) × 10−4
DV/K	1.4 ± 0.1	1.8 ± 0.1	n.a.
DV	1.4 ± 0.1	3.9 ± 0.1	n.a.
Dk2	n.a.	1.6 ± 0.1	3.8 ± 0.1
Dk3	n.a.	3.4 ± 0.1	4.0 ± 0.1

V/K reports on k_cat/K_m; V reports on k_cat; k₂, rate of acylation; k₃, rate of deacylation; n.a., not available;

^adata obtained from Reference 26.