Table 5.
Wildtype | Mutant | ||||||||
---|---|---|---|---|---|---|---|---|---|
Becker | OpenFLUX | FIA | Becker | OpenFLUX | FIA | ||||
const. | meas. | ratios | const. | meas. | |||||
Glucose 6-phosphate isomerase | 49.8 | 51.2 | 51.9 | 52.0 | 51.5 | 41.6 | 40.4 | 42.1 | 42.5 |
Glucose 6-phosphate dehydrogenase | 46.8 | 45.0 | 44.7 | 44.7 | 45.1 | 56.2 | 57.5 | 55.7 | 55.1 |
Transaldolase | 14 | 13.4 | 13.3 | 13.3 | 13.4 | 17.5 | 17.7 | 17.3 | 17.0 |
Transketolase 1 | 14 | 13.4 | 13.3 | 13.3 | 13.4 | 17.5 | 17.7 | 17.3 | 17.0 |
Transketolase 2 | 11.9 | 11.3 | 11.2 | 11.2 | 11.3 | 15.8 | 16.4 | 15.6 | 15.4 |
Glyceraldehyde 3-phosphate dehydrogenase | 157.5 | 158.0 | 158.2 | 158.6 | 158.0 | 160.8 | 161.0 | 161.0 | 160.5 |
Pyruvate kinase | 147.3 | 148.0 | 147.8 | 148.2 | 147.6 | 152.6 | 152.0 | 152.5 | 152.0 |
Pyruvate dehydrogenase | 77.5 | 75.8 | 74.8 | 74.9 | 74.9 | 87.5 | 85.2 | 85.1 | 79.7 |
Pyruvate carboxylase - carboxykinase | 34.4 | 35.8 | 35.9 | 36.1 | 35.8 | 31.5 | 32.4 | 32.5 | 34.9 |
Citrate synthase | 52.5 | 50.8 | 49.6 | 49.7 | 49.9 | 67.7 | 65.4 | 65.3 | 58.9 |
Isocitrate dehydrogenase | 52.5 | 50.8 | 49.6 | 49.7 | 49.9 | 67.7 | 65.4 | 65.3 | 58.9 |
Oxoglutarate dehydrogenase | 41.2 | 39.4 | 38.2 | 38.3 | 38.5 | 59.9 | 57.6 | 57.5 | 50.7 |
Aspartokinase | 11.2 | 11.2 | 11.2 | 11.4 | 11.2 | 14.2 | 14.2 | 14.2 | 15.9 |
Estimated metabolic fluxes values for the different approaches - the ad-hoc simulation results from Becker et al. [18], the OpenFLUX results [15], and the FIA results for its various simulated scenarios (measured fluxes used as constants, as measurements, and when using ratios of non-normalized data.)