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. Author manuscript; available in PMC: 2014 Jul 12.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jun 15;436(4):677–684. doi: 10.1016/j.bbrc.2013.06.016

Fig. 4.

Fig. 4

Trophoblast lineage cells (TC) derived from iPS cells (iPSC) differentiated into trophoblasts with 100ng/ml BMP4 for 7-day. (A) Multinuclear trophoblasts formed, nuclei marked by red. (B) CK-7 (green) positive cells. (C) Estradiol, hCG and Progesterone detected from D-7 culture medium. (n=3). (D) TC showed significant invasive capability compare to donor fibroblast in 20~22 hours invasion assay (n=3). Scale bar, 200μM.