FIGURE 9.

NaV1.1 current density is unaffected by CRMP2-K374A expression. A, exemplar family of current traces, in response to the voltage protocol shown in Fig. 8, from HEK293 cells expressing NaV1.1 and Ubc9, SUMO1 and CRMP2 (left), or CRMP2-K374A (right). B, summary of normalized peak currents recorded from NaV1.1-HEK293 cells expressing Ubc9, SUMO1, and CRMP2 (black bar) or CRMP2-K374A (white bar). Numbers in parentheses represent numbers of cells tested. There was no difference between the two conditions tested (p > 0.05, Student's t test). C, representative Boltzmann fits for activation and steady-state inactivation for NaV1.1-HEK293 cells transfected with the indicated constructs are shown. The calculated values for V_½ and k of activation and steady-state inactivation for all conditions tested and the associated statistics are presented in Table 2.