TABLE 1.
Comparative current densities, cell capacitances, and Boltzmann fits of voltage dependence of channel activation for the respective transfection conditions in CAD cells
Values for V½, the voltage of half-maximal activation, and slope were derived from Boltzmann distribution fits to the individual recordings and averaged to determine the mean ± S.E. As two cells were tested for the voltage dependence of activation for the condition with 3 CRMP2/1 CRMP2-K374A, only the average data are presented. N values are indicated in parentheses. All transfection conditions contained EGFP for identification of construct expression.
| Transfection condition | Peak current density | Cell capacitance | Voltage dependence of activation |
|
|---|---|---|---|---|
| V1/2 | Slope | |||
| pA/pF | pF | mV | mV/e-fold | |
| EGFP | 60.1 ± 19.3 (9) | 16.0 ± 2.5 (9) | −12.0 ± 1.0 (8) | 7.0 ± 0.7 (8) |
| CRMP2 | 63.7 ± 9.8 (11) | 21.9 ± 1.8 (11) | −9.9 ± 0.9 (11) | 5.7 ± 0.5 (11) |
| CRMP2-K374A | 19.1 ± 5.5 (10)a | 19.5 ± 2.0 (10) | −6.3 ± 1.6 (7)b | 7.6 ± 1.3 (7) |
| 3 CRMP2/1 CRMP2-K374A | 9.1 ± 3.1 (8)a,b | 15.7 ± 1.2 (8) | −13.7 (2) | 6.0 (2) |
| CRMP2AAA | 17.8 ± 4.9 (11)a,b | 17.9 ± 1.4 (11) | −10.6 ± 2.6 (4) | 7.2 ± 1.4 (4) |
| CRMP2 | 63.7 ± 9.8 (11) | 21.9 ± 1.8 (11) | −9.9 ± 0.9 (11) | 5.7 ± 0.5 (11) |
| CRMP2 + SENP1 | 16.0 ± 3.7 (10)a | 16.1 ± 1.8 (10) | −8.4 ± 1.1 (3) | 7.1 ± 0.9 (4) |
| CRMP2 + SENP2 | 14.6 ± 2.8 (11)a | 20.4 ± 1.4 (11) | −13.3 ± 1.6 (4) | 6.0 ± 1.2 (4) |
| CRMP2 + SENP1 + SENP2 | 20.0 ± 7.9 (12)a | 17.3 ± 1.6 (12) | −9.9 ± 1.7 (4) | 8.4 ± 1.0 (4) |
| CRMP2-K374A | 19.1 ± 5.5 (10) | 19.5 ± 2.0 (10) | −6.3 ± 1.6 (7) | 7.6 ± 1.3 (7) |
| CRMP2-K374A + SENP1 | 29.0 ± 12.2 (8) | 18.8 ± 3.2 (8) | −4.2 ± 1.9 (5) | 13.2 ± 1.2 (5)a |
| CRMP2-K374A + SENP2 | 14.7 ± 4.3 (8) | 16.2 ± 1.3 (8) | −3.5 ± 1.5 (3) | 9.2 ± 0.6 (3) |
| CRMP2-K374A + SENP1 + SENP2 | 26.9 ± 9.5 (9) | 21.4 ± 1.5 (9) | −3.4 ± 1.9 (3) | 11.0 ± 1.0 (3) |
| CRMP2AAA | 17.8 ± 4.9 (11) | 17.9 ± 1.4 (11) | −10.6 ± 2.6 (4) | 7.2 ± 1.4 (4) |
| CRMP2AAA + SENP1 + SENP2 | 16.0 ± 3.7 (11) | 15.4 ± 1.0 (11) | 1.2 ± 2.9 (4)* | 8.6 ± 1.6 (4) |
| EGFP | 60.1 ± 19.3 (9) | 16.0 ± 2.5 (9) | −12.0 ± 1.0 (8) | 7.0 ± 0.7 (8) |
| Ubc9 + SUMO1 | 49.8 ± 8.7 (10) | 14.4 ± 0.6 (10) | −9.8 ± 1.6 (9) | 8.3 ± 0.9 (9) |
a Data represent statistically significant differences as compared with control within the tested groups (p < 0.05, ANOVA with Tukey's post hoc test or Student's t test).
b Data denote statistical difference from EGFP control (p < 0.05, ANOVA with Tukey's post hoc test).