FIGURE 5.
FtsQ residues 250 and 255 are in close proximity to FtsB residues 88 and 77, respectively. The proximity of positions 232, 250, and 255 in FtsQ and positions 76, 77, 78, and 88 in FtsB was analyzed by cysteine cross-linking. Samples of cells expressing FtsL together with different combinations of FtsQ and FtsB variants, as indicated, were subjected to non-reducing SDS-PAGE and Western blotting using FtsQ-specific antibodies. Q, FtsQSH8; Q-B, FtsQSH8-FtsB adduct; asterisk, aspecifically detected bands.