Effects of altered CTCF activity on HCE and HTCE cell wound healing.
A, effect of overexpression (OE) of CTCF on corneal epithelial cell wound closure. B, significant acceleration of wound closure in CTCF-overexpressing cells. C, effect of knocking down CTCF on corneal epithelial cell wound closure. D, significant retardation of wound closure in CTCF knockdown cells. HCE and HTCE cells were infected with lentiviral vector containing either cDNA encoding full-length CTCF or CTCF-specific shRNA to overexpress or knock down CTCF, respectively. *, p < 0.05. Data were collected from four sets of independent experiments.