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. 2013 Jul 10;288(34):24363–24371. doi: 10.1074/jbc.M113.458141

FIGURE 4.

FIGURE 4.

Effect of suppressing CTCF on overexpression of NFκB p50-enhanced wound healing. A, effects of knocking down NFκB p50 mRNA and overexpression (OE) of p50 cDNA on CTCF activity. B, lack of EGF-induced CTCF expression in the corneas of NFκB p50−/− knockout mice detected by immunostaining. The corneas obtained from wild-type and NFκB p50−/− knockout mice with/without stimulation of EGF were compared in immunostaining sections. C, effect of knocking down CTCF on EGF-induced CTCF expression detected by Western blot analysis. D, effects of overexpressing NFκB p50 on wound closure in CTCF knockdown cells following a time course. E, a significant delay of NFκB p50 overexpression and EGF-induced wound closure by knockdown of CTCF. *, p < 0.05. Data were collected from four sets of independent experiments. Non-silencing (NS) shRNA was used as a control.