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. 2013 Jul 11;288(34):24382–24393. doi: 10.1074/jbc.M113.474676

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — PKD1-mediated phosphorylations of VASP are independent of each other. A, mimicking Ser-157 phosphorylation does not prime for Ser-322 phosphorylation. HeLa cells were transfected with GFP-VASP, GFP-VASP.S157E, or vector control as indicated. Cells were lysed and analyzed by Western blot for phosphorylation at Ser-322 using a phosphospecific antibody. VASP expression was controlled by re-probing with anti-GFP. B, mimicking Ser-322 phosphorylation does not prime for Ser-157 phosphorylation. HeLa cells were transfected with GFP-VASP, GFP-VASP.S322E, or vector control as indicated. Cells were lysed and analyzed by Western blot for phosphorylation at Ser-157 using a phosphospecific antibody. VASP expression was controlled by re-probing with anti-GFP.