FIGURE 5.

Binding of rCvGal1 to natural glycoproteins. A, binding of rCvGal1 to asialofetuin (20 μg/ml) in the presence of serial dilution of PSM, asialo-PSM (APSM), asialofetuin (ASF), BSM, asialo-BSM (ABSM), OSM, or asialo-OSM (AOSM) was measured in solid-based binding assay. Data show relative binding in triplicate with standard error. IC₅₀ values (50% inhibition concentrations) were calculated (Table 2). B, binding of rCvGal1 to untreated (Ctrl), or PNGase F-treated, or O-glycosidase (O-Gly'ase)-treated PSM-coated plates were measured. C, SPR was measured with immobilized PSM and rCvGal1 as analyte as for Fig. 4. SPR sensorgram was shown with inset as 1:1 steady state fitting curve. D, SPR was measured with immobilized asialofetuin and rCvGal1 as analyte. The surface plasmon resonance sensorgrams were recorded with 2-fold serial dilutions starting at the highest concentration labeled. SPR sensorgram was shown with inset as 1:1 steady state fitting curve.