FIGURE 9.

Binding of rCvGal1 to P. marinus trophozoites. A, binding of rCvGal1 (100 μg/ml) to P. marinus trophozoites was measured by flow cytometry analysis. Data show mean fluorescence intensity (MFI) ± S.E. of each sample. B, binding of rCvGal1 (100 μg/ml) to P. marinus trophozoites in the presence of PSM (0–100 μg/ml). * indicates significant difference (p < 0.05) from sample without PSM inhibition (0). Sample without exogenous rCvGal1 and inhibitor was shown (Ctrl). C, P. marinus trophozoites were stained with anti-blood group A antibody (red line), anti-blood group B antibody (yellow line), or control (black line) in flow cytometry analysis. D, P. marinus trophozoites were stained with fluorochrome-labeled lectins (red lines) or buffer alone (black lines) in flow cytometry analysis.