TABLE 2.

Contributions of free and bound R6G in the different complexes obtained from anisotropy decays analysis

All decays are fitted with the equation r(t) = r₀(a_f^r exp(−t/τ_f^r) + a_b^r exp(−t/τ_b^r)) + b by deconvoluting the instrument response function (IRF).

Sample	Fraction of free R6G (afr) with time-constant (τfr = 345 ps)	Fraction of bound R6G (abr) with time constant (τbr = 3.1 ns)	Residual anisotropy of bound R6G (b)	Total bound contribution (abr + b)
R6G in PBS	1	0	0	0
AD-CDR1	0.65	0.27	0.08	0.35
AD1–8u−	0.80	0.15	0.05	0.20
AD-CDR1 + farnesol	0.83	0.14	0.03	0.17
AD-CDR1+ ITC	0.75	0.14	0.04	0.18
V554A	0.83	0.16	0.01	0.17
M604A	0.79	0.19	0.02	0.21
I1237A	0.59	0.23	0.18	0.41
L1358A	0.60	0.31	0.09	0.40
L529A	0.80	0.20	0.01	0.21
V532A	0.83	0.17	0.001	0.17
C1294A	0.78	0.18	0.04	0.22