TABLE 3.

Fluorescence lifetime decay parameters of free and bound R6G in different complexes

All decays are fitted with the equation I(t) = a_f exp(−t/τ_f) + (a_b¹ exp(−t/τ_b¹) + a_b² exp(−t/τ_b²)) by deconvoluting the instrument response function (IRF).

Sample	Fraction of free R6G (af) with time-constant (τf = 3.9 ns)	Fraction of bound R6G (ab1) with time-constant (τb1=0.3 ns)	Fraction of bound R6G (ab2) with time-constant (τb2=1.8 ns)	Total contribution of bound R6G time-constants (ab1 + ab2)	Mean lifetime $\left(\langle \tau \rangle ={\displaystyle \sum _{i=f,b}{a}_i{\tau }_i}\right)$
R6G in PBS	1	0	0	0	3.91
AD-CDR1	0.15	0.54	0.31	0.85	1.31
AD1–8u−	0.36	0.35	0.29	0.64	2.03
AD-CDR1 + farnesol	0.38	0.33	0.29	0.62	2.10
AD-CDR1 + ITC	0.25	0.44	0.31	0.75	1.67
V554A	0.46	0.26	0.28	0.54	1.19
M604A	0.44	0.21	0.35	0.56	1.20
I1237A	0.11	0.53	0.35	0.88	1.26
L1358A	0.17	0.50	0.33	0.83	1.40
L529A	0.28	0.43	0.29	0.72	1.74
V532A	0.40	0.28	0.32	0.60	2.20
C1294A	0.34	0.40	0.26	0.66	1.91