FIGURE 5.

Mutation of the N-terminal CDK motifs results in enhanced Foxp3 expression and transcriptional function. A, CD4⁺25⁻ T lymphocytes were transduced with wild type or S/T→A(ble) Foxp3, restimulated as in Fig. 4, and Foxp3 and CD25 expression was assessed by flow cytometry. The mean fluorescence intensity (MFI) of each parameter was calculated and graphed. B, supernatants from restimulated cells in A were assessed for IL-2 production by ELISA. C, Il2 transcription normalized to actin is shown. Message level is represented in arbitrary units. The data shown are representative of at least two experiments. Error bars are S.E. for biological replicate cultures. **, p < 0.01, ***, p < 0.001.