FIGURE 3.

ANKRD2 negatively regulates myoblast differentiation program. pMB^WT and pMB^mdm cultured in GM were transfected with either pcDNA, pcDNA-ANKRD2-sense or pcDNA-ANKRD2-antisense vector for 48 h. A, knockdown of ANKRD2 protein was confirmed by Western blot in pMB^{mdm/Ankrd2↓}. B, knockdown of ANKRD2 promotes differentiation of pMB^{mdm/Ankrd2↓}, and co-transfection of ANKRD2-sense vector in pMB^{mdm/Ankrd2↓} voids the antisense ANKRD2-induced differentiation program. C, overexpression of ANKRD2 inhibits differentiation of pMB^WT/Ankrd2, and co-transfection of ANKRD2-antisense vector in pMB^WT/Ankrd2 voids the sense Ankrd2-induced differentiation program. D, overexpression of ANKRD2 protein was confirmed by Western blot in pMB^mdm/Ankrd2. Gel and immunocytochemistry pictures are representative of three separate experiments. ↑, overexpression; ↓, down-regulation.