TABLE 1.
Primers used in PCR
| Primer name | Sequence (5′–3′) | Underlined residues | Purpose |
|---|---|---|---|
| ANKRD2 sense (forward) | CCGGAATTCATGGAGGGTACCATGGAGGGG | EcoRI | Cloning |
| ANKNRD2 sense (reverse) | AACCCGGCTCGAGCGGCCGCGAGCAAAGCCAGCACTTTATTG | NotI | Cloning |
| ANKRD2 anti-sense (forward) | AACCCGGCTCGAGCGGCCGCATGGAGGGTACCATGGAGGG′ | EcoRI | Cloning |
| ANKNRD2 anti-sense (reverse) | CCGGAATTCAGCAAAGCCAGCACTTTATTG′ | NotI | Cloning |
| ID3 sense (forward) | CCGGAATTCTGTTTGCTGCTTTAGGTGTCTCTTTT | EcoRI | Cloning |
| ID3 sense (reverse) | CCCGGCTCGAGCGGCCGCATAAACCATTTTTGAACACTTTG | NotI | Cloning |
| ANKRD2 (forward) | GCCTCGGGGTTCAGAGTCCT | qPCR | |
| ANKRD2 (reverse) | GATCTCACGTCGCAGGTCCA | qPCR | |
| ID3 (forward) | ACCTGGAGCCCGAGAGAAGG | qPCR | |
| ID3 (reverse) | AGGGTGGGGACAGAGTGACG | qPCR |