FIGURE 2.

The production of IFNβ in Sendai virus infected MEFs is reduced in DEAF1−/− mice. A–I, immortalized MEFs from wild type mice (black bars, black circles in B) or DEAF1−/− mice (white bars, white squares in B) were infected with Sendai virus (100 HA/ml) for the times indicated. Total RNA was extracted and analyzed by qRT-PCR to measure the mRNA encoding IFNβ, IRF7, IFNα4, IFNα6, CXCL10, IκBα, IL-12p40, and the Sendai virus Pgene. The results show fold increase in mRNA relative to the values measured in unstimulated MEFs (± S.D. for triplicate determinations). In panel B, the concentration of IFNβ in the culture medium was measured by ELISA. The results are shown ± S.D. for triplicate determinations. Similar results were obtained in four independent experiments using MEFs immortalized from two different wild type and two different DEAF1−/− mice.