FIGURE 8.

RNA interference of DEAF1 suppresses IFNβ mRNA production in poly(I:C)-stimulated HEK293-TLR3 cells. HEK293 cells stably expressing the TLR3 receptor (termed HEK293-TLR3 cells) were transfected with “scrambled” siRNA (control) or with the indicated amounts of DEAF1-blocking siRNA. 72 h later, the cells were stimulated with poly(I:C) (50 μg/ml) for the times indicated, total RNA extracted and the mRNA encoding DEAF1 (A), IFNβ (B), and Pellino1 (C) was measured by qRT-PCR as described under “Experimental Procedures.” The results show relative mRNA levels (± S.D. for triplicate determinations) compared with the value of 1.0 measured in unstimulated control, HEK293-TLR3 cells. The experiment was performed in 12-well plates with three wells used for each condition. Similar results were obtained in two independent experiments.